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5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches

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Nucleic Acid Detection and Structural Investigations

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2063))

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Abstract

Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.

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Acknowledgments

The work was partially supported by Russian State funded budget project # АААА-А17-117020210021-7 to ICBFM SB RAS.

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia

    D. S. Novopashina & A. G. Venyaminova

  2. Novosibirsk State University, Novosibirsk, Russia

    D. S. Novopashina & O. A. Semikolenova

Authors
  1. D. S. Novopashina
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  2. O. A. Semikolenova
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  3. A. G. Venyaminova
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Corresponding author

Correspondence to D. S. Novopashina .

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Editors and Affiliations

  1. Department of Chemistry, Technical University of Denmark, Kongens Lyngby, Denmark

    Kira Astakhova

  2. Department of Chemistry, Technical University of Denmark, Kongens Lyngby, Denmark

    Syeda Atia Bukhari

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Novopashina, D.S., Semikolenova, O.A., Venyaminova, A.G. (2020). 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. In: Astakhova, K., Bukhari, S. (eds) Nucleic Acid Detection and Structural Investigations. Methods in Molecular Biology, vol 2063. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0138-9_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0138-9_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0137-2

  • Online ISBN: 978-1-0716-0138-9

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