Abstract
This chapter reports a library preparation protocol for efficient high-throughput sequencing of double-stranded RNA viruses. The protocol consists of four main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing adapters for high-throughput sequencing. This protocol will be useful for all double-stranded RNA viruses and for all of the high-throughput sequencing platforms.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Taxonomy V (2017) The classification and nomenclature of viruses. The online (10th) report of the ICTV. Arch Virol 51(1–2):141–149
Aly M, Al Khairy A, Al Johani S, Balkhy H (2015) Unusual rotavirus genotypes among children with acute diarrhea in Saudi Arabia. BMC Infect Dis 15(1):192. https://doi.org/10.1186/s12879-015-0923-y
Shi J, Hu Z, Deng F, Shen S (2018) Tick-borne viruses. Virol Sin 33(1):21–43. https://doi.org/10.1007/s12250-018-0019-0
Diarra M, Fall M, Fall AG, Diop A, Lancelot R, Seck MT, .Guis H. (2018). Spatial distribution modelling of Culicoides (Diptera: Ceratopogonidae) biting midges, potential vectors of African horse sickness and bluetongue viruses in Senegal. Parasit Vectors, 11(1):341. doi: https://doi.org/10.1186/s13071-018-2920-7
Bournez L, Cavalerie L, Sailleau C, Breard E, Zanella G., de Almeida RS, .Hendrikx P. (2018). Estimation of French cattle herd immunity against bluetongue serotype 8 at the time of its re-emergence in 2015. BMC Vet Res, 14(1):65. doi: https://doi.org/10.1186/s12917-018-1388-1
Maan S, Rao S, Maan NS, Anthony SJ, Attoui H, Samuel AR, Mertens PPC (2007) Rapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses. J Virol Methods 143(2):132–139. https://doi.org/10.1016/j.jviromet.2007.02.016
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Dolgova, A.S., Safonova, M.V., Dedkov, V.G. (2020). Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses. In: Astakhova, K., Bukhari, S. (eds) Nucleic Acid Detection and Structural Investigations. Methods in Molecular Biology, vol 2063. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0138-9_14
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0138-9_14
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0137-2
Online ISBN: 978-1-0716-0138-9
eBook Packages: Springer Protocols