Abstract
The study of the population of muscle satellite cells (SC) is important to understand muscle regeneration and its involvement in the different dystrophic processes. We studied two dystrophic mouse models, Largemyd and Lama2dy2j/J, that show an intense and very similar pattern of muscle degeneration, but with differences in the expression of genes involved in the regeneration cascade. They are, therefore, interesting models to study possible differences in the mechanism of activation and action of satellite cells in the dystrophic muscle. The main objectives of this chapter are to describe the isolation and characterization of SC populations, evaluating the presence of myogenic and pluripotent stem cells markers in normal and dystrophic muscles.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Morgan JE, Partridge TA (2003) Muscle satellite cells. Int J Biochem Cell Biol 35:1151–1156
Chen JC, Goldhamer DJ (2003) Skeletal muscle stem cells. Reprod Biol Endocrinol 1:101
Hawke TJ, Garry DJ (2001) Myogenic satellite cells: physiology to molecular biology. J Appl Physiol 91:534–551
Kallestad KM, McLoon LK (2010) Defining the heterogeneity of skeletal muscle-derived side and main population cells isolated immediately ex vivo. J Cell Physiol 222:676–684
Scott IC, Tomlinson W, Walding A et al (2013) Large-scale isolation of human skeletal muscle satellite cells from post-mortem tissue and development of quantitative assays to evaluate modulators of myogenesis. J Cachexia Sarcopenia Muscle 4:157–169
Tajika Y, Takahashi M, Hino M et al (2010) VAMP2 marks quiescent satellite cells and myotubes, but not activated myoblasts. Acta Histochem Cytochem 43:107–114
Yajima H, Motohashi N, Ono Y et al (2010) Six family genes control the proliferation and differentiation of muscle satellite cells. Exp Cell Res 316:2932–2944
Li Y, Pan H, Huard J (2010) Isolating stem cells from soft musculoskeletal tissues. J Vis Exp 41:pii:2011
Jankowski RJ, Deasy BM, Huard J (2002) Muscle-derived stem cells. Gene Ther 9:642–647
Shefer G, de Mark DP, Richardson JB, Yablonka-Reuveni Z (2006) Satellite-cell pool size does matter: defining the myogenic potency of aging skeletal muscle. Dev Biol 294:50–66
Seale P, Sabourin LA, Girgis-Gabardo A et al (2000) Pax7 is required for the specification of myogenic satellite cells. Cell 102:777–786
Zammit PS, Golding JP, Nagata Y et al (2004) Muscle satellite cells adopt divergent fates: a mechanism for self-renewal? J Cell Biol 166:347–357
Shefer G, Rauner G, Yablonka-Reuveni Z, Benayahu D (2010) Reduced satellite cell numbers and myogenic capacity in aging can be alleviated by endurance exercise. PLoS One 5:e13307
Halevy O, Piestun Y, Allouh MZ et al (2004) Pattern of Pax7 expression during myogenesis in the posthatch chicken establishes a model for satellite cell differentiation and renewal. Dev Dyn 231:489–502
Lindström M, Thornell LE (2009) New multiple labelling method for improved satellite cell identification in human muscle: application to a cohort of power-lifters and sedentary men. Histochem Cell Biol 132:141–157
Dominici M, Le Blanc K, Mueller I et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells: the International Society for cellular therapy position statement. Cytotherapy 8:315–317
Patki S, Kadam S, Chandra V, Bhonde R (2010) Human breast milk is a rich source of multipotent mesenchymal stem cells. Hum Cell 23:35–40
Acknowledgments
This work was made possible thanks to financial support from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), CNPq, and CAPES.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Onofre-Oliveira, P.C.G., Vainzof, M. (2020). Isolation and Characterization of Muscle-Derived Stem Cells from Dystrophic Mouse Models. In: Astakhova, K., Bukhari, S. (eds) Nucleic Acid Detection and Structural Investigations. Methods in Molecular Biology, vol 2063. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0138-9_13
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0138-9_13
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0137-2
Online ISBN: 978-1-0716-0138-9
eBook Packages: Springer Protocols