Abstract
The vast majority of immunoassays carried out fall into the category of enzyme immunosorbent assays. These are routinely used for the diagnosis of infectious agents such as viruses and other substances in blood. The antigen is the substance or agent to be measured. In this technique the antigen is immobilized onto a solid phase, either a reaction vessel or a bead.
An antigen is a substance capable of causing an immune response leading to the production of antibodies, and it is also a target to which antibodies will bind. Antibodies are antigen specific and will only bind to the antigen that initiated their production. The purpose of this chapter is to discuss antibody and antigen reactions that will be focused on various immuno-techniques.
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Abbreviations
- BSA:
-
Bovine serum albumin
- FACS:
-
Fluorescence-activated cell sorter analysis
- FCA:
-
Freund’s complete adjuvant
- FIA:
-
Freund’s incomplete adjuvant
- ISCOMs:
-
Immune-stimulating complexes
- SPIEM:
-
Solid-phase immune electron microscopy
- SRID:
-
Single radial immunodiffusion
References
Rajan K (2011) Analytical techniques in biochemistry and molecular biology. Springer, New York. eBook ISBN978-1-4419-9785-2
Wilson K, Walker J (2010) Principles and techniques of biochemistry and molecular biology. Cambridge University Press, Cambridge. ISBN 978-0-521-73167-6
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Basha, M. (2020). Immuno-techniques. In: Analytical Techniques in Biochemistry. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0134-1_7
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DOI: https://doi.org/10.1007/978-1-0716-0134-1_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0133-4
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