Skip to main content
SpringerLink
Log in

Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility

  • Protocol
  • First Online:
Ion Mobility-Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2084))

Abstract

Matrix-Assisted Laser Desorption Ionization (MALDI) and Desorption Electrospray Ionization (DESI) are two complementary ionization techniques that have transformed the field of biomolecular analysis, enabling the measurement of a wide range of biomolecules by mass spectrometry. These techniques have also been applied to imaging mass spectrometry where the spatial localization of molecules is determined. Coupling this with Ion Mobility Spectrometry (IM) allows an additional level of separation and specificity to be obtained. Here, we describe the coupling of the technologies and the practical advantages of these combinations, highlighting specific examples.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 149.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Hillenkamp F, Karas M, Beavis RC, Chait BT (1991) Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers. Anal Chem 63(24):1193A–1203A

    Article  CAS  Google Scholar 

  2. Yamashita M, Fenn JB (1984) Electrospray ion source. Another variation on the free-jet theme. J Phys Chem 88(20):4451–4459

    Article  CAS  Google Scholar 

  3. Henzel WJ, Billeci TM, Stults JT, Wong SC, Grimley C, Watanabe C (1993) Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases. Proc Natl Acad Sci U S A 90(11):5011–5015

    Article  CAS  Google Scholar 

  4. Cain TC, Lubman DM, Weber WJ Jr (1994) Differentiation of bacteria using protein profiles from matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 8:1026–1030

    Article  CAS  Google Scholar 

  5. Caprioli RM, Farmer TB, Gile J (1997) Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS. Anal Chem 69:4751

    Article  CAS  Google Scholar 

  6. Takáts Z, Wiseman JM, Gologan B, Cooks RG (2004) Mass spectrometry sampling under ambient conditions with desorption electrospray ionization. Science 306(5695):471–473

    Article  Google Scholar 

  7. Ifa DR, Wiseman JM, Song QY, Cooks RG (2007) Development of capabilities for imaging mass spectrometry under ambient conditions with desorption electrospray ionization (DESI). Int J Mass Spectrom 259:8–15

    Article  CAS  Google Scholar 

  8. Kallback P, Shariatgorji M, Nilsson A, Andren PE (2012) Novel mass spectrometry imaging software assisting labeled normalization and quantitation of drugs and neuropeptides directly in tissue sections. J Proteome 75:4941–4951

    Article  Google Scholar 

  9. Chaurand P, Schwartz SA, Caprioli RM (2002) Imaging mass spectrometry: a new tool to investigate the spatial organization of peptides and proteins in mammalian tissue sections. Curr Opin Chem Biol 6:676–681

    Article  CAS  Google Scholar 

  10. Goodwin RJ, Mackay CL, Nilsson A, Harrison DJ, Farde L, Andren PE et al (2011) Qualitative and quantitative MALDI imaging of the positron emission tomography ligands raclopride (a D2 dopamine antagonist) and SCH 23390 (a D1 dopamine antagonist) in rat brain tissue sections using a solvent-free dry matrix application method. Anal Chem 83:9694–9701

    Article  CAS  Google Scholar 

  11. Ibrahim YM, Prior DC, Baker ES, Smith RD, Belov ME (2010) Characterization of an ion mobility-multiplexed collision induced dissociation-tandem time-of-flight mass spectrometry approach. Int J Mass Spectrom 293(1–3):34–44

    Article  CAS  Google Scholar 

  12. Rodriguez-Suarez E, Hughes C, Gethings L, Giles K, Wildgoose J, Stapels M, Fadgen K, Geromanos S,Vissers JPC, Elortza F, Langridge JI (2013) An Ion Mobility Assisted Data Independent LC-MS Strategy for the Analysis of Complex Biological Samples. Current Analytical Chemistry 9(2):199–211.

    CAS  Google Scholar 

  13. Vakhrushev SY, Langridge J, Campuzano I, Hughes C, Peter-Katalinić J (2008) Ion mobility mass spectrometry analysis of human glycourinome. Anal Chem 80(7):2506–2513

    Article  CAS  Google Scholar 

  14. Paglia G, Williams JP, Menikarachchi L, Thompson JW, Tyldesley-Worster R, Halldórsson S, Rolfsson O, Moseley A, Grant D, Langridge J, Palsson BO, Astarita G (2014) Ion mobility derived collision cross sections to support metabolomics applications. Anal Chem 86(8):3985–3993

    Article  CAS  Google Scholar 

  15. May JC, McLean JA (2015) Ion mobility-mass spectrometry: time-dispersive instrumentation. Anal Chem 87(3):1422–1143

    Article  CAS  Google Scholar 

  16. Ewing MA, Glover MS, Clemmer DE (2016) Hybrid ion mobility and mass spectrometry as a separation tool. J Chromatogr A 1439:3–25

    Article  CAS  Google Scholar 

  17. Pringle SD, Giles K, Wildgoose JL, Williams JP, Slade SE, Thalassinos K, Bateman RH, Bowers MT, Scrivens J (2007) An investigation of the mobility separation of some peptide and protein ions using a new hybrid quadrupole/travelling wave IMS/OA-ToF instrument. Int J Mass Spectrom 261:1–12

    Article  CAS  Google Scholar 

  18. Giles K, Pringle SD, Worthington KR, Little D, Wildgoose JL, Bateman RH (2004) Applications of a travelling wave-based radio-frequency-only stacked ring ion guide. Rapid Commun Mass Spectrom 18(20):2401–2414

    Article  CAS  Google Scholar 

  19. Powers TW, Neely BA, Shao Y, Tang H, Troyer DA, Mehta AS, Haab BB, Drake RR (2014) MALDI imaging mass spectrometry profiling of N-glycans in formalin-fixed paraffin embedded clinical tissue blocks and tissue microarrays. PLoS One 9:9

    Google Scholar 

  20. Nelson KA, Daniels GJ, Fournie JW, Hemmer MJ (2013) Optimization of whole-body zebrafish sectioning methods for mass spectrometry imaging. J Biomol Tech 24(3):119–127

    Article  Google Scholar 

  21. Shariatgorji M, Svenningsson P, Andrén PE (2014) Mass spectrometry imaging, an emerging technology in neuropsychopharmacology. Neuropsychopharmacology 39:34–49

    Article  CAS  Google Scholar 

  22. Djidja MC, Francese S, Loadman PM, Sutton CW, Scriven P, Claude E, Snel MF, Franck J, Salzet M, Clench MR (2009) Detergent addition to tryptic digests and ion mobility separation prior to MS/MS improves peptide yield and protein identification for in situ proteomic investigation of frozen and formalin-fixed paraffin-embedded adenocarcinoma tissue sections. Proteomics 9(10):2750–2763

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Waters Corporation, Wilmslow, UK

    James I. Langridge & Emmanuelle Claude

Authors
  1. James I. Langridge
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Emmanuelle Claude
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to James I. Langridge .

Editor information

Editors and Affiliations

  1. Department of Medicine and Surgery, University of Milano-Bicocca, Vedano al Lambro, Monza e Brianza, Italy

    Giuseppe Paglia

  2. Georgetown University, Washington, DC, USA

    Giuseppe Astarita

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Langridge, J.I., Claude, E. (2020). Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility. In: Paglia, G., Astarita, G. (eds) Ion Mobility-Mass Spectrometry . Methods in Molecular Biology, vol 2084. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0030-6_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-0030-6_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0029-0

  • Online ISBN: 978-1-0716-0030-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation