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Ion Mobility-Mass Spectrometry of Glycoconjugates

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Ion Mobility-Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2084))

Abstract

Glycoconjugates are diverse biomolecules that are dynamically assembled to regulate and fine-tune numerous cellular processes. Their biosynthesis is nontemplate-driven, achieved stepwise in discrete locations within the cell, giving rise to a range of complex branched structures that pose a significant challenge in structural biology. Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures. In this chapter, we briefly discuss recent applications of IM for glycomics and describe how to acquire, interpret, and analyze IM-MS data for the analysis of glycans.

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Author information

Authors and Affiliations

  1. Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK

    Weston B. Struwe

  2. Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford, UK

    Weston B. Struwe

  3. Nuffield Department of Medicine, Target Discovery Institute, University of Oxford, Oxford, UK

    David J. Harvey

Authors
  1. Weston B. Struwe
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  2. David J. Harvey
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Corresponding author

Correspondence to Weston B. Struwe .

Editor information

Editors and Affiliations

  1. Department of Medicine and Surgery, University of Milano-Bicocca, Vedano al Lambro, Monza e Brianza, Italy

    Giuseppe Paglia

  2. Georgetown University, Washington, DC, USA

    Giuseppe Astarita

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Struwe, W.B., Harvey, D.J. (2020). Ion Mobility-Mass Spectrometry of Glycoconjugates. In: Paglia, G., Astarita, G. (eds) Ion Mobility-Mass Spectrometry . Methods in Molecular Biology, vol 2084. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0030-6_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0030-6_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0029-0

  • Online ISBN: 978-1-0716-0030-6

  • eBook Packages: Springer Protocols

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