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Compositional Characterization of Acidic Petroleum Constituents Using Negative Ion Mode Electrospray Ionization Coupled with Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry

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Hydrocarbon and Lipid Microbiology Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Direct infusion Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS) is an emerging tool in the characterization of complex mixtures of organic compounds such as petroleum fluids. Ultra-high mass resolution is achieved based on the extremely precise determination of cyclotron frequencies of ions forced on orbits in an ion trap by a high homogenous magnetic field. The achievable mass resolution depends on the strength of the magnetic field. In this protocol we present a method for compositional characterization of acidic petroleum constituents using negative ion mode electrospray ionization coupled with FT-ICR MS. The protocol describes sample preparation, measurement, and data evaluation and presents some examples for the visualization of the complex data sets obtained. We do so by comparing results for two crude oil samples from offshore Norway, one of which has experienced in-reservoir biodegradation while the other has not. Pitfalls of the used methodology and alternative ionization methods are briefly addressed.

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Acknowledgements

We would like to thank the Norwegian Petroleum Directorate for providing the crude oil samples analyzed in the study.

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Authors and Affiliations

  1. Helmholtz Centre Potsdam (GFZ) German Research Centre for Geosciences, Telegrafenberg, D-14473, Potsdam, Germany

    Stefanie Poetz

  2. Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University, Carl-von-Ossietzky-Straße 9-11, D-26111, Oldenburg, Germany

    Heinz Wilkes

Authors
  1. Stefanie Poetz
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  2. Heinz Wilkes
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Corresponding author

Correspondence to Stefanie Poetz .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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Poetz, S., Wilkes, H. (2016). Compositional Characterization of Acidic Petroleum Constituents Using Negative Ion Mode Electrospray Ionization Coupled with Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry. In: McGenity, T., Timmis, K., Nogales, B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2016_203

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  • DOI: https://doi.org/10.1007/8623_2016_203

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-52791-7

  • Online ISBN: 978-3-662-52793-1

  • eBook Packages: Springer Protocols

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