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Characterization of Bacterial Symbionts in Deep-Sea Fauna: Protocols for Sample Conditioning, Fluorescence In Situ Hybridization, and Image Analysis

  • Sébastien DuperronEmail author
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Symbioses with bacteria are key adaptations allowing various groups of metazoans to reach high biomasses at deep sea reducing habitats including hydrothermal vents and cold seeps. Characterizing these associations is challenging due to the constraints associated with work on deep-sea organisms. These include limited sample availability, impact of recovery procedures and shipment on sample quality, and general lack of environmental data. In this chapter, a standard procedure for sample processing at sea which can maximize sample use back in the laboratory is presented, with example protocols for sample fixation, fluorescence in situ hybridization (FISH)-based localization of symbionts in animal tissues, and estimation of their relative abundances in the case of multiple symbioses.

Keywords

Deep sea Fluorescence in situ hybridization Sample conditioning Symbiosis 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Sorbonne Universités, UPMC Université Paris 06, UMR 7208 BOREA, Adaptation aux Milieux ExtrêmesParisFrance
  2. 2.Institut Universitaire de FranceParisFrance

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