Abstract
Microorganisms utilizing hydrocarbons anaerobically as organic growth substrates are grown in defined anoxic media with sulfate, nitrate, or other electron acceptors. Cultivation of such microorganisms is technically often more elaborate than that of other anaerobes. Special technical measures include, for instance, dilution of potentially toxic hydrocarbons in an inert carrier phase and precautions to avoid diffusion of traces of oxygen through stoppers during long incubations. Furthermore, contact of liquid hydrocarbons with stoppers should be avoided.
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Widdel, F. (2015). Cultivation of Anaerobic Microorganisms with Hydrocarbons as Growth Substrates. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2015_186
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DOI: https://doi.org/10.1007/8623_2015_186
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