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Oleaginous Microalgae Isolation and Screening for Lipid Productivity Using a Standard Protocol

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Hydrocarbon and Lipid Microbiology Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Microalgae have great potential to serve as a feedstock for biodiesel, based on their high areal productivity and their ability to accumulate large amounts of triacylglycerols. In contrary to first-generation biofuel crops, microalgae do not need to compete with arable land or biodiverse landscapes and can be grown in virtually any type of water, including fresh, brackish, saline and wastewater. The use of local microalgal strains is desirable, as they are often dominant under the local geographical, climatic and ecological conditions. Here we describe a user-friendly method that enables the isolation of monoalgal strains, coupled to a standard protocol to evaluate their potential for lipid accumulation by directly comparing lipid productivities. Other important criteria to consider include the ability to harvest microalgae and extract their oil cost-effectively.

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Acknowledgement

We wish to thank Meat and Livestock Australia and the Endeavour Foundation for financial support.

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Authors and Affiliations

  1. Algae Biotechnology Laboratory, School of Agriculture and Food Sciences, The University of Queensland, Brisbane, 4072, Australia

    Van Thang Duong, Boer Bao & Peer M. Schenk

Authors
  1. Van Thang Duong
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  2. Boer Bao
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  3. Peer M. Schenk
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Corresponding author

Correspondence to Peer M. Schenk .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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© 2015 Springer-Verlag Berlin Heidelberg

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Duong, V.T., Bao, B., Schenk, P.M. (2015). Oleaginous Microalgae Isolation and Screening for Lipid Productivity Using a Standard Protocol. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2015_181

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  • DOI: https://doi.org/10.1007/8623_2015_181

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-45178-6

  • Online ISBN: 978-3-662-45179-3

  • eBook Packages: Springer Protocols

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