Measurements of Hydrocarbon Pollutants in Aqueous Samples Using Bacterial Bioreporter Assays
Bacterial bioreporters are useful tools for analyzing the bioavailability and/or toxic effects of hydrocarbons, especially in aqueous samples. Bioreporters generally consist of a recombinant organism in which a genetic circuit is placed that serves to produce an easily detectable reporter protein in response to contact with the hydrocarbon. In this part, we will mainly focus on reporter assays for short-chain linear alkanes and aromatic solvents with two different inducible reporter proteins (e.g., LuxAB luciferase and EGFP or enhanced green fluorescent protein). We will outline a few procedures on the types of assays that can be performed with the bacterial bioreporters in water samples.
KeywordsBacteria Bioreporter Inductions Pollutants
This work was supported by grants from the EC-FP7 program (BRAAVOO, OCEAN-2013-614010) and the Swiss National Science Foundation Nano-Tera program (Envirobot, 20NA21-143082).
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