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Protocols for the Measurement of Bacterial Chemotaxis to Hydrocarbons

  • Jayna L. Ditty
  • Rebecca E. ParalesEmail author
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Bacterial chemotaxis is the process by which bacteria sense and respond to environmental stimuli. While the mechanism for chemotaxis has been extensively studied in enteric bacteria, studies in soil bacteria that are attracted to aromatic acids and aromatic hydrocarbons in addition to sugars and amino acids are lacking. Here we describe detailed protocols for the quantitative and qualitative assessment of chemotaxis responses to analyze responses to hydrocarbon and aromatic acid attractants to identify the specific receptors involved.

Keywords:

Aromatic hydrocarbons Attractant Chemotaxis Energy taxis Methyl-accepting chemotaxis protein 

Notes

Acknowledgments

We thank Rita Luu, Jonathan Hughes, Benjamin Schneider, Janet Rollefson, and undergraduate students that participated in the BIOL464 Bioinformatics course at the University of St. Thomas for providing data and figures and Rita Luu for critically reading the manuscript. Chemotaxis studies were supported by a grant from the National Science Foundation to REP and JLD (MCB0919930). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of BiologyUniversity of St. ThomasSt. PaulUSA
  2. 2.Department of Microbiology and Molecular GeneticsThe University of CaliforniaDavisUSA

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