Abstract
Thermodynamics is central to our understanding of the ecology and physiology of microbial ecosystems. In physiological research, thermodynamics is used to determine the directionality of a reaction or the feasibility of a pathway. In ecological research, thermodynamics is used to determine the feasibility of a process and to rationalize the sequence of redox reactions in both natural environments and engineered biological systems and increasingly also as a basis to describe microbial activities as a function of environmental factors. This protocol provides a detailed annotated description of how to calculate change in Gibbs free energy values and redox potentials and a brief discussion on linking thermodynamics and kinetics for reactions of biological interest.
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Dolfing, J. (2015). Protocols for Calculating Reaction Kinetics and Thermodynamics. In: McGenity, T., Timmis, K., Nogales Fernández, B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2015_109
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DOI: https://doi.org/10.1007/8623_2015_109
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