Fluorescence Microscopy for Microbiology
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Fluorescence microscopy allows selective recognition of a particular component from biomolecular complex structures for the investigation of biological processes. It is frequently used to image specific microbial features. The rapid development of new fluorescent probes that can be easily adapted for a wide array of biological applications, coupled to the extraordinary technical improvements in microscope systems and software, strongly supports the sustained development and exploitation of fluorescence microscopy as a powerful research tool.
Fluorescence microscopy in microbiology can investigate the localization and levels of molecules and can provide information about their distribution, dynamics, and interactions, both in living and fixed samples. The preparation of high-quality samples for microscopic observation is the starting point for obtaining good resolution and optimum imaging results. This chapter attempts to provide basic methods for the application of conventional fluorescence and immunofluorescence microscopy for microbiology.
Keywords:Fluorescence microscopy Fluorochrome Fluorophore Immunofluorescence
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