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Protocols for the Isolation and Preliminary Characterization of Bacteria for Biodesulfurization and Biodenitrogenation of Petroleum-Derived Fuels

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Hydrocarbon and Lipid Microbiology Protocols

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Abstract

The use of microorganisms to improve fuel quality has been proposed, and in this chapter we describe basic experimental procedures for the isolation and characterization of bacteria for biodesulfurization and biodenitrogenation with dibenzothiophene and carbazole as model compounds for each application, respectively. The presented protocols should be considered as a starting point and adapted to other problematic compounds and conditions. Basic protocols for the evaluation of the treatment of real oil fractions are also presented.

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Notes

  1. 1.

    ASTM D4629-08 Standard test method for trace nitrogen in liquid petroleum hydrocarbons by syringe/inlet oxidative combustion and chemiluminescence detection

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Authors and Affiliations

  1. Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Avenida Vasco de Quiroga 4871, Col. Santa Fe Cuajimalpa, CP 05300, México DF, Mexico

    Marcia Morales & Sylvie Le Borgne

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  1. Marcia Morales
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  2. Sylvie Le Borgne
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Correspondence to Sylvie Le Borgne .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex Dept. Biological Sciences, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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Morales, M., Le Borgne, S. (2014). Protocols for the Isolation and Preliminary Characterization of Bacteria for Biodesulfurization and Biodenitrogenation of Petroleum-Derived Fuels. In: McGenity, T., Timmis, K., Nogales, B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2014_41

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  • DOI: https://doi.org/10.1007/8623_2014_41

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-53113-6

  • Online ISBN: 978-3-662-53115-0

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