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Protocols for the Isolation and Analysis of Lipopeptides and Bioemulsifiers

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Hydrocarbon and Lipid Microbiology Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

High molecular weight biosurfactants are produced from a number of different bacteria and comprise lipoproteins, proteins, polysaccharides, lipopolysaccharides or complexes containing several of these structural types, many of which have yet to be fully characterised. Lipopeptide biosurfactants are cyclic peptides with varying attached lipid chains and are most commonly isolated from Bacillus and Pseudomonas strains. A great deal of research has been carried out on these cyclic lipopeptides, and their structures have been fully characterised. The ability to isolate, purify and characterise these structures is extremely important, providing detailed information with regard to different cultivation condition and biological activities. Similar methods can be used for both lipopeptides and biopolymers especially when attempting to determine their amino acid sequences. The experimental techniques used to isolate, purify and analyse these biosurfactants are widely varied from simple colorimetric assays giving an approximate indication of the type of compounds present to the more complex mass spectrometric techniques that provide information on molecular mass and structural features. Mass spectrometry provides essential information in the identification of these structures using sophisticated MS/MS analysis and software technologies. The use of Edman degradation can help confirm the results observed from de novo sequencing experiments, thus providing further confirmation of amino acid sequence of both lipopeptides and protein-containing biopolymers. This chapter details information on the most commonly reported techniques used to analyse these types of biosurfactants.

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Author information

Authors and Affiliations

  1. Department of Life Sciences, School of Science, Institute of Technology Sligo, Co. Sligo, Republic of Ireland

    Thomas J. Smyth

  2. School of Biomedical Sciences, University of Ulster, Coleraine, Londonderry, Northern Ireland, BT52 1SA, UK

    Michelle Rudden, Konstantina Tsaousi, Roger Marchant & Ibrahim M. Banat

Authors
  1. Thomas J. Smyth
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  2. Michelle Rudden
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  3. Konstantina Tsaousi
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  4. Roger Marchant
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  5. Ibrahim M. Banat
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Corresponding author

Correspondence to Ibrahim M. Banat .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Dept. Biology, University of the Balearic Islands and University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)", Palma de Mallorca, Spain

    Balbina Nogales

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© 2014 Springer-Verlag Berlin Heidelberg

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Smyth, T.J., Rudden, M., Tsaousi, K., Marchant, R., Banat, I.M. (2014). Protocols for the Isolation and Analysis of Lipopeptides and Bioemulsifiers. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols . Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2014_29

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  • DOI: https://doi.org/10.1007/8623_2014_29

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-49135-5

  • Online ISBN: 978-3-662-49137-9

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