Protocol for Inferring Compound Biodegradation at Low Concentrations from Biomass Measurements

  • Siham Beggah
  • Jan Roelof van der MeerEmail author
Part of the Springer Protocols Handbooks book series (SPH)


Biodegradation tests for organic compounds are usually performed at relatively high carbon concentrations (2–100 mg L−1), which can be problematic for toxic compounds. Here we describe a protocol to test compound biodegradation through the concomitant formation of bacterial biomass at relatively low carbon concentrations (0.5–5 mg C/L). The protocol is based on accurate cell counting of dilute cell suspensions by flow cytometry coupled to cell staining with fluorescent dyes.


Bacterial population growth Cell staining Flow cytometry 



This work was supported by grants from the EC-FP7 program (BACSIN, KBBE-211684) and the Swiss National Science Foundation Nano-Tera program (Envirobot, 20NA21-143082).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of Fundamental Microbiology, Bâtiment BiophoreUniversity of LausanneLausanneSwitzerland

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