Protocol for Inferring Compound Biodegradation at Low Concentrations from Biomass Measurements
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Biodegradation tests for organic compounds are usually performed at relatively high carbon concentrations (2–100 mg L−1), which can be problematic for toxic compounds. Here we describe a protocol to test compound biodegradation through the concomitant formation of bacterial biomass at relatively low carbon concentrations (0.5–5 mg C/L). The protocol is based on accurate cell counting of dilute cell suspensions by flow cytometry coupled to cell staining with fluorescent dyes.
KeywordsBacterial population growth Cell staining Flow cytometry
This work was supported by grants from the EC-FP7 program (BACSIN, KBBE-211684) and the Swiss National Science Foundation Nano-Tera program (Envirobot, 20NA21-143082).
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