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Hydrocarbon and Lipid Microbiology Protocols pp 81–91Cite as

Isolation and Characterization of Lipid Droplets from Yeast

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Abstract

Lipid droplets are cell compartments serving as a depot for non-polar lipids, mainly triacylglycerols and steryl esters. Furthermore, these compartments are involved in lipid metabolism, signal transduction, membrane biosynthesis and trafficking, as well as storage of proteins, and additional functions may be discovered in the future. A crucial prerequisite for detailed studies of lipid droplets is the availability of a highly purified cell fraction. Here, a protocol for the isolation of lipid droplets from the model organism yeast Saccharomyces cerevisiae is presented. Characteristics of a lipid droplet fraction are a limited number of proteins and a high content of non-polar lipids. Most common problems occurring during isolation of lipid droplets and advices to overcome these problems are included.

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Acknowledgement

This work has been financially supported by the Austrian Science Fund (FWF; Projects 21251 and P26308 to K.A.).

Author information

Authors and Affiliations

  1. Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, 8010, Graz, Austria

    Karin Athenstaedt

  2. Institute of Biochemistry, Graz University of Technology, Petersgasse 12/2, 8010, Graz, Austria

    Karin Athenstaedt

Authors
  1. Karin Athenstaedt
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Corresponding author

Correspondence to Karin Athenstaedt .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Dept. Biology, University of the Balearic Islands and University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)", Palma de Mallorca, Spain

    Balbina Nogales

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© 2014 Springer-Verlag Berlin Heidelberg

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Athenstaedt, K. (2014). Isolation and Characterization of Lipid Droplets from Yeast. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols . Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2014_2

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  • DOI: https://doi.org/10.1007/8623_2014_2

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-49135-5

  • Online ISBN: 978-3-662-49137-9

  • eBook Packages: Springer Protocols

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