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Protocol for the Measurement of Hydrocarbon Transport in Bacteria

  • Jayna L. Ditty
  • Nancy N. Nichols
  • Rebecca E. ParalesEmail author
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Due to the hydrophobicity, volatility, and relatively low aqueous solubility of aliphatic and aromatic hydrocarbons, transport of these chemicals by bacteria has not been extensively studied. These issues make transport assays difficult to carry out, and as a result, strong evidence for the active transport of hydrocarbons is lacking. Here we describe a detailed protocol for the measurement of hydrocarbon transport in bacteria and suggest key equipment and control experiments required to obtain convincing results.

Keywords

Aromatic hydrocarbons Metabolic inhibitors Radiolabeled substrates Specific activity Transport 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Jayna L. Ditty
    • 1
  • Nancy N. Nichols
    • 2
  • Rebecca E. Parales
    • 3
    Email author
  1. 1.Department of BiologyUniversity of St. ThomasSt. PaulUSA
  2. 2.U.S. Department of AgricultureNational Center for Agricultural Utilization Research, Agricultural Research ServicePeoriaUSA
  3. 3.Department of Microbiology and Molecular GeneticsThe University of CaliforniaDavisUSA

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