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Hydrocarbon and Lipid Microbiology Protocols pp 55–67Cite as

Protocol for the Measurement of Hydrocarbon Transport in Bacteria

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Abstract

Due to the hydrophobicity, volatility, and relatively low aqueous solubility of aliphatic and aromatic hydrocarbons, transport of these chemicals by bacteria has not been extensively studied. These issues make transport assays difficult to carry out, and as a result, strong evidence for the active transport of hydrocarbons is lacking. Here we describe a detailed protocol for the measurement of hydrocarbon transport in bacteria and suggest key equipment and control experiments required to obtain convincing results.

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Author information

Authors and Affiliations

  1. Department of Biology, University of St. Thomas, St. Paul, MN, USA

    Jayna L. Ditty

  2. U.S. Department of Agriculture, National Center for Agricultural Utilization Research, Agricultural Research Service, Peoria, IL, USA

    Nancy N. Nichols

  3. Department of Microbiology and Molecular Genetics, The University of California, Davis, CA, USA

    Rebecca E. Parales

Authors
  1. Jayna L. Ditty
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  2. Nancy N. Nichols
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  3. Rebecca E. Parales
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Corresponding author

Correspondence to Rebecca E. Parales .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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© 2014 Springer-Verlag Berlin Heidelberg

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Ditty, J.L., Nichols, N.N., Parales, R.E. (2014). Protocol for the Measurement of Hydrocarbon Transport in Bacteria. In: McGenity, T., Timmis, K., Nogales, B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2014_11

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  • DOI: https://doi.org/10.1007/8623_2014_11

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-49138-6

  • Online ISBN: 978-3-662-49140-9

  • eBook Packages: Springer Protocols

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