Protocols for Measuring Biosurfactant Production in Microbial Cultures

  • Roger Marchant
  • Ibrahim M. BanatEmail author
Part of the Springer Protocols Handbooks book series (SPH)


Microbial biosurfactants have wide structural and functional diversity which consequently requires the adoption of a range of techniques to investigate these amphiphilic molecules. Literature on the production and analytical detection of biosurfactants is overwhelmed with assertions of high yields for such products which are mostly over exaggerated estimates due to the use of flawed or inaccurate analytical techniques. In this chapter we focus on quantitative methods available to allow accurate estimates of production and yield data to be generated.


Colorimetric methods HPLC/MS Rhamnolipid Sophorolipid Surface/interfacial tension UPLC 



We would like to thank Dr Thomas J.P. Smyth and Mr Scot Funston for valuable comments and results from analytical methods.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.School of Biomedical Sciences, University of UlsterNorthern IrelandUK

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