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Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes

  • Kaur Jaanson
  • Angela Pärn
  • Tõnis TimmuskEmail author
Protocol
Part of the Neuromethods book series (NM, volume 143)

Abstract

BDNF gene has a complex structure and is regulated by multiple time- and stimulus-dependent promoters and distal regulatory elements. To better understand the interplay between these regulatory units, it is beneficial to study BDNF regulation in the context of a larger gene locus. The bacterial artificial chromosome (BAC) system allows propagating up to 300 kb long genomic regions, enabling to study the whole BDNF gene. Here, we present the methods for usage of BACs for studying BDNF gene regulation in primary cultures of rat cortical neurons and astrocytes.

Keywords

Astrocytes Bacterial artificial chromosome BDNF Neurons Primary culture Transfection 

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Department of Chemistry and BiotechnologyTallinn University of TechnologyTallinnEstonia

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