Abstract
The understanding of brain function requires the study of the cellular and molecular mechanisms that govern the growth and development of neuron connections. This is a process driven by the polarization of the neuron into a highly structured morphology with dendritic arborizations and a long axonal projection. In recent years, miRNAs have been described as key players in these processes, both at the level of axon development and synaptic plasticity. Here, we describe a microfluidic compartmentalized cortical neuron culture and the in utero electroporation techniques for the study of miRNAs and their role in axon development.
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Acknowledgments
We thank Dr. Yolanda Saavedra Torres for the comments on the manuscript and Wai Han Wong for technical support.
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Garcez, P.P., Guillemot, F., Dajas-Bailador, F. (2016). Study of miRNA Function in the Developing Axons of Mouse Cortical Neurons: Use of Compartmentalized Microfluidic Chambers and In Utero Electroporation. In: Kye, M. (eds) MicroRNA Technologies. Neuromethods, vol 128. Humana Press, New York, NY. https://doi.org/10.1007/7657_2016_12
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DOI: https://doi.org/10.1007/7657_2016_12
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7173-2
Online ISBN: 978-1-4939-7175-6
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