Integrated Optogenetic and Electrophysiological Dissection of Local Cortical Circuits In Vivo

  • Jessica A. Cardin
Part of the Neuromethods book series (NM, volume 67)


The recent development of optical methods for controlling the activity of specific populations of neurons holds tremendous promise for understanding the roles of neural subtypes in local circuits, a major goal of systems neuroscience. Optogenetic tools, in combination with electrophysiology, allow identification and manipulation of specific groups of excitatory and inhibitory neurons in active neural networks in vivo in a spatially and temporally precise manner. Here, we provide a detailed description of methods for integrating optogenetics with more traditional electrophysiological approaches as a means to probe these complex interactions.

Key words

Optogenetic Interneuron Oscillation Channelrhodopsin-2 Halorhodopsin Artifact Light-evoked activity Cortex Electrophysiology In vivo 



This work was funded by NIH/NEI R00 EY018407, the Whitehall Foundation, and the Esther A. and Joseph Klingenstein Foundation.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Jessica A. Cardin
    • 1
  1. 1.Department of NeurobiologyYale University School of MedicineNew HavenUSA

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