Combining Optical Stimulation with Extracellular Electrophysiology in Behaving Mice

  • Joshua H. Siegle
Part of the Neuromethods book series (NM, volume 67)


Optical manipulation allows researchers to alter the activity of genetically defined neural populations with millisecond-scale temporal accuracy. This facilitates the activation and inactivation of functionally distinct subnetworks at precise times relative to behavior, something that was not previously possible. This chapter describes the construction of an implant for simultaneous light delivery and extracellular electrophysiology in unanesthetized, unrestrained mice. The implant contains eight stereotrodes surrounding a central fiber optic cable, is compact and lightweight, and can be modified for other recording/stimulation configurations.

Key words

Optogenetics Channelrhodopsin Stereotrodes Chronic recording Behavior Mice 



I would like to thank members of the laboratories of Matthew Wilson, Christopher Moore, and Susumu Tonegawa for advice on in vivo electrophysiology and optogenetics. This work was supported by an NDSEG Fellowship.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Joshua H. Siegle
    • 1
  1. 1.Department of Brain and Cognitive SciencesMassachusetts Institute of TechnologyCambridgeUSA

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