Combining Optical Stimulation with Extracellular Electrophysiology in Behaving Mice
Optical manipulation allows researchers to alter the activity of genetically defined neural populations with millisecond-scale temporal accuracy. This facilitates the activation and inactivation of functionally distinct subnetworks at precise times relative to behavior, something that was not previously possible. This chapter describes the construction of an implant for simultaneous light delivery and extracellular electrophysiology in unanesthetized, unrestrained mice. The implant contains eight stereotrodes surrounding a central fiber optic cable, is compact and lightweight, and can be modified for other recording/stimulation configurations.
Key wordsOptogenetics Channelrhodopsin Stereotrodes Chronic recording Behavior Mice
I would like to thank members of the laboratories of Matthew Wilson, Christopher Moore, and Susumu Tonegawa for advice on in vivo electrophysiology and optogenetics. This work was supported by an NDSEG Fellowship.
- 13.Zorzos AN, Dietrich A et al (2009) Light-proof neural recording electrodes. Soc Neurosci (Abstract)Google Scholar
- 17.Zorzos AN, Bernstein JG et al (2009) Integrated microstructure lightguides for ultradense optical neural control of 3-dimensional neural circuits. Soc Neurosci (Abstract)Google Scholar
- 18.Ritt JT, Siegle JH et al (2009) Optical manipulation of cortical subnetworks during active touch in awake mice using channelrhodopsin-2. Soc Neurosci (Abstract)Google Scholar
- 19.Kvitsiani D, Ranade S et al (2009) Optical tagging of genetically defined cell types for extracellular recording in behaving rodents. Soc Neurosci (Abstract)Google Scholar
- 22.Oliveira LMO, Dimitrov D (2008) Surgical techniques for chronic implantation of microwire arrays in rodents and primates. In: Nicolelis MAL (ed) Methods for neural ensemble recording, 2nd edn. CRC, Boca Raton, FLGoogle Scholar
- 23.Dzirasa K (2008) Chronic recordings in transgenic mice. In: Nicolelis MAL (ed) Methods for neural ensemble recording, 2nd edn. CRC, Boca Raton, FLGoogle Scholar