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Measuring the Messenger: RNA Histology in Formalin-Fixed Tissues

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Molecular Histopathology and Tissue Biomarkers in Drug and Diagnostic Development

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

RNA in situ hybridization (ISH) complements immunohistochemistry (IHC) by confirming localized gene expression in a tissue. Effective use of ISH requires careful communication and coordination between molecular biologists, histologists, and pathologists. This chapter reviews the literature and experience of the authors in the use of formalin-fixed paraffin-embedded tissues for RNA histology techniques.

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Author information

Authors and Affiliations

  1. Flagship Biosciences, 2995 Wilderness Place, Suite 105, Boulder, CO, 80301, USA

    Steven J. Potts, Mirza Peljto, Mahipal Suraneni & Joseph S. Krueger

Authors
  1. Steven J. Potts
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  2. Mirza Peljto
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  3. Mahipal Suraneni
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  4. Joseph S. Krueger
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Corresponding author

Correspondence to Steven J. Potts .

Editor information

Editors and Affiliations

  1. Flagship Biosciences, LLC, Westminster, Colorado, USA

    Steven J. Potts

  2. University of North Carolina, Chapel Hill, North Carolina, USA

    David A. Eberhard

  3. Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA

    Keith A. Wharton, Jr.

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© 2014 Springer Science+Business Media New York

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Potts, S.J., Peljto, M., Suraneni, M., Krueger, J.S. (2014). Measuring the Messenger: RNA Histology in Formalin-Fixed Tissues. In: Potts, S., Eberhard, D., Wharton, Jr., K. (eds) Molecular Histopathology and Tissue Biomarkers in Drug and Diagnostic Development. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/7653_2014_19

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  • DOI: https://doi.org/10.1007/7653_2014_19

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2680-0

  • Online ISBN: 978-1-4939-2681-7

  • eBook Packages: Springer Protocols

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