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Molecular Imaging of Therapeutic Effect of Mesenchymal Stem Cell-Derived Exosomes for Hindlimb Ischemia Treatment

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Imaging and Tracking Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2150))

Abstract

Critical limb ischemia is a major cause of morbidity and mortality worldwide. Recently, many studies confirmed that MSC-derived exosomes (MSC-exosomes) had potential therapeutic effect to treat hindlimb ischemia through pro-angiogenesis. The therapeutic angiogenesis is a critical measurement to judge the beneficial effect of MSC-exosomes treatment. Formerly, the therapeutic effect of MSC-exosomes was usually evaluated through clinical assessment and histopathological examination. Here, we describe a strategy to evaluate the therapeutic effect of MSC-exosomes by monitoring the therapeutic angiogenesis with bioluminescent imaging in hindlimb ischemia mice models.

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Acknowledgments

This research was supported by National Key R&D Program of China (2017YFA0103200), National Natural Science Foundation of China (81671734), Key Projects of Tianjin Science and Technology Support Program (18YFZCSY00010), Fundamental Research Funds for the Central Universities (63181114).

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Authors and Affiliations

  1. Nankai University School of Medicine, Tianjin, China

    Kaiyue Zhang & Zongjin Li

  2. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China

    Kaiyue Zhang & Zongjin Li

  3. The Key Laboratory of Bioactive Materials, Ministry of Education, The College of Life Science, Nankai University, Tianjin, China

    Zongjin Li

Authors
  1. Kaiyue Zhang
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  2. Zongjin Li
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Corresponding author

Correspondence to Zongjin Li .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Zhang, K., Li, Z. (2019). Molecular Imaging of Therapeutic Effect of Mesenchymal Stem Cell-Derived Exosomes for Hindlimb Ischemia Treatment. In: Turksen, K. (eds) Imaging and Tracking Stem Cells. Methods in Molecular Biology, vol 2150. Humana, New York, NY. https://doi.org/10.1007/7651_2019_221

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  • DOI: https://doi.org/10.1007/7651_2019_221

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0626-1

  • Online ISBN: 978-1-0716-0627-8

  • eBook Packages: Springer Protocols

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