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pp 1-6 | Cite as

Total Nitrogen Determination by a Spectrophotometric Method

  • Jaana Koistinen
  • Mervi Sjöblom
  • Kristian Spilling
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Being able to measure total nitrogen (TN) is important for following the nitrogen budget. In this chapter, we present the spectrophotometric method we use for determining TN. The method relies on oxidation and reduction steps, involving persulfate digestion of nitrogen compounds into nitrate followed by spectrophotometric determination.

Keywords

Nutrient uptake Total nitrogen 

References

  1. 1.
    Grasshoff K, Ehrhardt M, Kremling K (1999) Methods of seawater analysis. Wiley-VCH Verlag Gmbh, WeinheimGoogle Scholar
  2. 2.
    Schnetger B, Lehners C (2014) Determination of nitrate plus nitrite in small volume marine water samples using vanadium(III)chloride as a reduction agent. Mar Chem 160:91–98Google Scholar
  3. 3.
    Koroleff F (1979) Methods for the chemical analysis for seawater. Meri 7:1–60. (in Finnish)Google Scholar

Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Jaana Koistinen
    • 1
  • Mervi Sjöblom
    • 1
  • Kristian Spilling
    • 2
  1. 1.Tvärminne Zoological StationUniversity of HelsinkiHankoFinland
  2. 2.Finnish Environment InstituteHelsinkiFinland

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