Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering

  • Bing-Dong Sui
  • Bin Zhu
  • Cheng-Hu Hu
  • Pan Zhao
  • Yan JinEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2002)


The niche plays critical roles in regulating functionality and determining regenerative outcomes of stem cells, for which establishment of favorable microenvironments is in demand in translational medicine. In recent years, the cell aggregate technology has shown immense potential to reconstruct a beneficial topical niche for stem cell-mediated regeneration, which has been recognized as a promising concept for high-density stem cell delivery with preservation of the self-produced, tissue-specific extracellular matrix microenvironments. Here, we describe the basic methodology of stem cell aggregate-based niche engineering and quality check indexes prior to application.


Cell aggregate Extracellular matrix Microenvironment Niche engineering Stem cells Tissue regeneration 



This work was supported by grants from the National Key Research and Development Program of China (2016YFC1102900 and 2016YFC1101400), the General Program of National Natural Science Foundation of China (81570937 and 81470710), and the State Scholarship Fund of China (201603170205).


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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Bing-Dong Sui
    • 1
    • 2
  • Bin Zhu
    • 1
    • 2
  • Cheng-Hu Hu
    • 1
    • 2
    • 3
  • Pan Zhao
    • 1
    • 2
  • Yan Jin
    • 1
    • 2
    • 3
    Email author
  1. 1.State Key Laboratory of Military Stomatology, Center for Tissue Engineering, School of StomatologyFourth Military Medical UniversityXi’anChina
  2. 2.Research and Development Center for Tissue EngineeringFourth Military Medical UniversityXi’anChina
  3. 3.Xi’an Institute of Tissue Engineering and Regenerative MedicineXi’anChina

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