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Identification and Characterization of Stem Cells in Oral Cancer

  • Sujit K. BhutiaEmail author
  • Prajna P. Naik
  • Prakash P. Praharaj
  • Debasna P. Panigrahi
  • Chandra S. Bhol
  • Kewal K. Mahapatra
  • Sarbari Saha
  • Srimanta Patra
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2002)

Abstract

Cancer stem cells (CSCs) are a subpopulation of cells within a heterogeneous tumor that have enhanced biologic properties such as increased capacity for self-renewal, increased tumorigenicity, enhanced differentiation capacity, and resistance to chemo- and radiotherapies. This unit describes protocols to isolate and characterize potential cancer stem cells from a solid tumor (oral cancer). This involves creating a single-cell suspension from tumor tissue, tagging the cell subpopulation of interest, and sorting cells into different populations. Finally, the sorted subpopulations can be evaluated for their ability to meet the functional requirements of a CSC, which primarily include increased tumorigenicity in an in vivo xenograft assay. Mastering the protocols in this unit will allow the researcher to study populations of cells that may have properties of CSCs.

Keywords

Aldehyde dehydrogenase activity Cancer stem cells CD44 Orosphere Side population Xenograft 

Notes

Acknowledgments

Research support was partly provided by Board of Research in Nuclear Sciences (BRNS) [Number: 37(1)/14/38/2016-BRNS/37276], Department of Atomic Energy (DAE), and Science and Engineering Research Board (SERB) [Number: EMR/2016/001246]. Research infrastructure was partly provided by Fund for Improvement of S&T Infrastructure in Universities and Higher Educational Institutions (FIST) [Number: SR/FST/LSI-025/2014], Department of Science and Technology, Government of India.

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Sujit K. Bhutia
    • 1
    Email author
  • Prajna P. Naik
    • 1
    • 2
  • Prakash P. Praharaj
    • 1
  • Debasna P. Panigrahi
    • 1
  • Chandra S. Bhol
    • 1
  • Kewal K. Mahapatra
    • 1
  • Sarbari Saha
    • 1
  • Srimanta Patra
    • 1
  1. 1.Department of Life ScienceNational Institute of Technology RourkelaRourkelaIndia
  2. 2.PG Department of Zoology, Vikram Deb (Auto) CollegeJeyporeIndia

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