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Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche

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Stem Cell Niche

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2002))

Abstract

Adult skeletal muscle stem cells, termed satellite cells, are essential for regenerating muscle after tissue damage. Satellite cells are located in a specialized microenvironment between muscle fibers and their surrounding basal lamina. This local niche serves as a compartment to preserve satellite cell function and provides signals that facilitate the rapid response to injury. Visualization of this local niche enables the elucidation of such niche-derived signals. Here, we describe techniques for isolating single myofibers with their associated satellite cells for ex vivo visualization and analysis of an intact muscle stem cell niche.

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Acknowledgments

We thank D. Cornelison, A. Brack, and C. Crist for gifts of reagents and helpful advice. This work was supported by NIH grants AR046207 and AR070231 to R.S.K. and by the Tisch Cancer Institute at Mount Sinai NIH P30 CA196521 for support of work on the Mount Sinai Microscopy CoRE.

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Authors and Affiliations

  1. Department of Cell, Developmental, and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Aviva J. Goel & Robert S. Krauss

Authors
  1. Aviva J. Goel
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  2. Robert S. Krauss
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Corresponding author

Correspondence to Robert S. Krauss .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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Goel, A.J., Krauss, R.S. (2018). Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche. In: Turksen, K. (eds) Stem Cell Niche. Methods in Molecular Biology, vol 2002. Humana, New York, NY. https://doi.org/10.1007/7651_2018_177

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  • DOI: https://doi.org/10.1007/7651_2018_177

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9507-3

  • Online ISBN: 978-1-4939-9508-0

  • eBook Packages: Springer Protocols

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