Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche

  • Aviva J. Goel
  • Robert S. KraussEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2002)


Adult skeletal muscle stem cells, termed satellite cells, are essential for regenerating muscle after tissue damage. Satellite cells are located in a specialized microenvironment between muscle fibers and their surrounding basal lamina. This local niche serves as a compartment to preserve satellite cell function and provides signals that facilitate the rapid response to injury. Visualization of this local niche enables the elucidation of such niche-derived signals. Here, we describe techniques for isolating single myofibers with their associated satellite cells for ex vivo visualization and analysis of an intact muscle stem cell niche.


Cadherin Catenin Cell adhesion Integrin Muscle Niche Quiescence Regeneration Satellite cell Stem cell 



We thank D. Cornelison, A. Brack, and C. Crist for gifts of reagents and helpful advice. This work was supported by NIH grants AR046207 and AR070231 to R.S.K. and by the Tisch Cancer Institute at Mount Sinai NIH P30 CA196521 for support of work on the Mount Sinai Microscopy CoRE.


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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Department of Cell, Developmental, and Regenerative BiologyIcahn School of Medicine at Mount SinaiNew YorkUSA

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