Analysis of Hematopoietic Niche in the Mouse Embryo

  • Keai Sinn Tan
  • Nathalie Brouard
  • Daisuke SugiyamaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2002)


The development, differentiation, and maturation of hematopoietic cells are regulated by the intrinsic and extrinsic regulation. Intrinsic activity is affected by cell autonomous gene expression and extrinsic factors originate from the so-called niche surrounding the hematopoietic cells. It remains unclear why the hematopoietic sites are shifted during embryogenesis. Flow cytometry and immunohistochemistry enable us to study embryonic regulation of hematopoietic niche in the mouse embryo.


Embryo Hematopoietic Mouse Niche 



We thank the Ministry of Education, Culture, Sports, Science and Technology; The Ministry of Health, Labor and Welfare; and the Japan Society for the Promotion of Science for grant support.


  1. 1.
    Sugiyama D, Inoue-Yokoo T, Fraser ST, Kulkeaw K, Mizuochi C, Horio Y (2011) Embryonic regulation of the mouse hematopoietic niche. ScientificWorldJournal 11:1770–1780CrossRefGoogle Scholar
  2. 2.
    Swain A, Inoue T, Tan KS, Nakanishi Y, Sugiyama D (2014) Intrinsic and extrinsic regulation of mammalian hematopoiesis in the fetal liver. Histol Histopathol 29:1077–1082PubMedGoogle Scholar
  3. 3.
    Suire C, Brouard N, Hirschi K, Simmons PJ (2012) Isolation of the stromal-vascular fraction of mouse bone marrow markedly enhances the yield of clonogenic stromal progenitors. Blood 119:e86–e95CrossRefGoogle Scholar
  4. 4.
    Sasaki T, Mizuochi C, Horio Y, Nakao K, Akashi K, Sugiyama D (2010) Regulation of hematopoietic cell clusters in the placental niche through SCF/Kit signaling in embryonic mouse. Development 137:3941–3952CrossRefGoogle Scholar
  5. 5.
    Sasaki T, Tanaka Y, Kulkeaw K, Yumine-Takai A, Tan KS, Nishinakamura R, Ishida J, Fukamizu A, Sugiyama D (2016) Embryonic intra-aortic clusters undergo myeloid differentiation mediated by mesonephros-derived CSF1 in mouse. Stem Cell Rev 12:530–542CrossRefGoogle Scholar
  6. 6.
    Sugiyama D, Kulkeaw K, Mizuochi C, Horio Y, Okayama S (2011) Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production. Biochem Biophys Res Commun 410:301–306CrossRefGoogle Scholar
  7. 7.
    Sugiyama D, Kulkeaw K, Mizuochi C (2013) TGF-beta-1 up-regulates extra-cellular matrix production in mouse hepatoblasts. Mech Dev 130:195–206CrossRefGoogle Scholar
  8. 8.
    Brouard N, Jost C, Matthias N, Albrecht C, Egard S, Gandhi P, Strassel C, Inoue T, Sugiyama D, Simmons PJ et al (2017) A unique microenvironment in the developing liver supports the expansion of megakaryocyte progenitors. Blood Adv 1:1854–1866CrossRefGoogle Scholar
  9. 9.
    Tan KS, Kulkeaw K, Nakanishi Y, Sugiyama D (2017) Expression of cytokine and extracellular matrix mRNAs in fetal hepatic stellate cells. Genes Cells 22:836–844CrossRefGoogle Scholar
  10. 10.
    Tan KS, Inoue T, Kulkeaw K, Tanaka Y, Lai MI, Sugiyama D (2015) Localized SCF and IGF-1 secretion enhances erythropoiesis in the spleen of murine embryos. Biol Open 4:596–607CrossRefGoogle Scholar
  11. 11.
    Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y, Sugiyama D (2015) Embryonic hematopoietic progenitor cells reside in muscle before bone marrow hematopoiesis. PLoS One 10:e0138621CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Keai Sinn Tan
    • 1
  • Nathalie Brouard
    • 2
  • Daisuke Sugiyama
    • 3
    • 4
    • 5
    • 6
    • 7
    Email author
  1. 1.Institute of Biomedical and Pharmaceutical SciencesGuangdong University of TechnologyGuangzhouChina
  2. 2.Unité Mixte de Recherche - S1255 Recherche Plaquettes Sanguines: Hémostase, Thrombose, TransfusionUniversité de Strasbourg, INSERM, Etablissement Français du Sang-Alsace, Fédération de Médecine Translationnelle de StrasbourgStrasbourgFrance
  3. 3.Department of Research and Development of Next Generation Medicine, Faculty of Medical SciencesKyushu UniversityFukuokaJapan
  4. 4.Center for Clinical and Translational ResearchKyushu University HospitalFukuokaJapan
  5. 5.Incubation Center for Advanced Medical ScienceKyushu UniversityFukuokaJapan
  6. 6.Department of Clinical Study, Center for Advanced Medical InnovationKyushu UniversityFukuokaJapan
  7. 7.Kyushu University, Station for Collaborative Research1 4FFukuokaJapan

Personalised recommendations