Functional Assays of Stem Cell Properties Derived from Different Niches
It has been described that adult tissues contain mesenchymal stem cell populations. The specific areas where stem cells reside are known as niches. Crosstalk between cells and their niche is essential to maintain the correct functionality of stem cell. MSCs present a set of abilities such as migration, invasion, and angiogenic potentials, which make them ideal candidates for cell-based therapies. In order to test the regenerative capacity of these cells, we have described a methodology for the collection and for the evaluation of these mesenchymal precursors from different niches.
KeywordsAngiogenesis Explant Invasion Mesenchymal stem cell Migration Niche
This study was supported by the grant from the Spanish Ministry of Science and Innovation (SAF 2015–67911-R) to BGG. BdL is supported by FPU fellowships from the Spanish Ministry of Science and Innovation.
BdL and LMP wrote the manuscript. BGG revised the manuscript. All authors read and approved the final manuscript. We would like to thank all past and present members of the “BGG group”, who have contributed to the design and evolution of these protocols over several years.
- 2.Galvez BG, Sampaolesi M, Barbuti A, Crespi A, Covarello D, Brunelli S, Dellavalle A, Crippa S, Balconi G, Cuccovillo I, Molla F, Staszewsky L, Latini R, Difrancesco D, Cossu G (2008) Cardiac mesoangioblasts are committed, self-renewable progenitors, associated with small vessels of juvenile mouse ventricle. Cell Death Differ 15(9):1417–1428. https://doi.org/10.1038/cdd.2008.75 CrossRefPubMedGoogle Scholar
- 6.Weidenhamer NK, Moore DL, Lobo FL, Klair NT, Tranquillo RT (2015) Influence of culture conditions and extracellular matrix alignment on human mesenchymal stem cells invasion into decellularized engineered tissues. J Tissue Eng Regen Med 9(5):605–618. https://doi.org/10.1002/term.1974 CrossRefPubMedPubMedCentralGoogle Scholar