Advertisement

Functional Assays of Stem Cell Properties Derived from Different Niches

  • Beatriz de Lucas
  • Laura M. PérezEmail author
  • Beatriz G. Gálvez
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2002)

Abstract

It has been described that adult tissues contain mesenchymal stem cell populations. The specific areas where stem cells reside are known as niches. Crosstalk between cells and their niche is essential to maintain the correct functionality of stem cell. MSCs present a set of abilities such as migration, invasion, and angiogenic potentials, which make them ideal candidates for cell-based therapies. In order to test the regenerative capacity of these cells, we have described a methodology for the collection and for the evaluation of these mesenchymal precursors from different niches.

Keywords

Angiogenesis Explant Invasion Mesenchymal stem cell Migration Niche 

Notes

Acknowledgments

This study was supported by the grant from the Spanish Ministry of Science and Innovation (SAF 2015–67911-R) to BGG. BdL is supported by FPU fellowships from the Spanish Ministry of Science and Innovation.

BdL and LMP wrote the manuscript. BGG revised the manuscript. All authors read and approved the final manuscript. We would like to thank all past and present members of the “BGG group”, who have contributed to the design and evolution of these protocols over several years.

References

  1. 1.
    Bernal A, Fernandez M, Perez LM, San Martin N, Galvez BG (2012) Method for obtaining committed adult mesenchymal precursors from skin and lung tissue. PLoS One 7(12):e53215.  https://doi.org/10.1371/journal.pone.0053215 CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Galvez BG, Sampaolesi M, Barbuti A, Crespi A, Covarello D, Brunelli S, Dellavalle A, Crippa S, Balconi G, Cuccovillo I, Molla F, Staszewsky L, Latini R, Difrancesco D, Cossu G (2008) Cardiac mesoangioblasts are committed, self-renewable progenitors, associated with small vessels of juvenile mouse ventricle. Cell Death Differ 15(9):1417–1428.  https://doi.org/10.1038/cdd.2008.75 CrossRefPubMedGoogle Scholar
  3. 3.
    Galvez BG, San Martin N, Rodriguez C (2009) TNF-alpha is required for the attraction of mesenchymal precursors to white adipose tissue in Ob/ob mice. PLoS One 4(2):e4444.  https://doi.org/10.1371/journal.pone.0004444 CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Hass R, Kasper C, Bohm S, Jacobs R (2011) Different populations and sources of human mesenchymal stem cells (MSC): a comparison of adult and neonatal tissue-derived MSC. Cell Commun Signal 9:12.  https://doi.org/10.1186/1478-811X-9-12 CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    De Becker A, Riet IV (2016) Homing and migration of mesenchymal stromal cells: how to improve the efficacy of cell therapy? World J Stem Cells 8(3):73–87.  https://doi.org/10.4252/wjsc.v8.i3.73 CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Weidenhamer NK, Moore DL, Lobo FL, Klair NT, Tranquillo RT (2015) Influence of culture conditions and extracellular matrix alignment on human mesenchymal stem cells invasion into decellularized engineered tissues. J Tissue Eng Regen Med 9(5):605–618.  https://doi.org/10.1002/term.1974 CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Tao H, Han Z, Han ZC, Li Z (2016) Proangiogenic features of mesenchymal stem cells and their therapeutic applications. Stem Cells Int 2016:1314709.  https://doi.org/10.1155/2016/1314709 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Beatriz de Lucas
    • 1
    • 2
  • Laura M. Pérez
    • 1
    • 2
    Email author
  • Beatriz G. Gálvez
    • 1
    • 2
  1. 1.Universidad Europea de MadridMadridSpain
  2. 2.Instituto de Investigación Hospital 12 de Octubre (I+12)MadridSpain

Personalised recommendations