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Skin Stem Cells pp 385–392Cite as

Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

Human hair follicle-associated pluripotent (hHAP) stem cells were isolated from the upper parts of human hair follicles. hHAP stem cells were suspended in DMEM containing 10% fetal bovine serum (FBS) where they differentiated to cardiac muscle cells as well as neurons, glial cells, keratinocytes, and smooth muscle cells. The methods of this chapter are appropriate for use of human hair follicles to produce hHAP stem cells in sufficient quantities for future heart, nerve, and spinal cord regeneration in the clinic.

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References

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Author information

Authors and Affiliations

  1. AntiCancer Inc., San Diego, CA, USA

    Robert M. Hoffman

  2. Department of Surgery, University of California, San Diego, San Diego, CA, USA

    Robert M. Hoffman

Authors
  1. Robert M. Hoffman
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Correspondence to Robert M. Hoffman .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Hoffman, R.M. (2018). Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_170

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  • DOI: https://doi.org/10.1007/7651_2018_170

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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