Abstract
Mitochondrial reactive oxygen species (mROS), a major source of ROS within cells, functions as an important signaling molecule and has the ability to damage cellular macromolecules including DNA and proteins. Monitoring mROS levels is therefore essential to understand cell–cell communication and programmed cell death in all types of cell including stem cells. Here, we describe generation and characterization of a redox sensor for mROS that is specifically expressed in the germline stem cells (GSCs) in Drosophila. This redox sensor can be used to monitor the production of mROS and mitophagy in the GSCs during oogenesis.
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Acknowledgments
We thank Arundhati Bali for technical assistance and members of the Shravage lab for helpful discussions. We thank Dr. K.M. Paknikar, Director, Agharkar Research Institute, Pune, and entire Developmental Biology fraternity for support and access to facilities. This work was supported by grants from DBT-Ramalingaswami Fellowship and DST-SERB grant number ECR/2015/000239. B.V.S. is affiliated to Savitribai Phule Pune University (SPPU), Pune, India and is recognized by SPPU as PhD guide (Biotechnology). KSN is affiliated to Department of Biotechnology, Savitribai Phule Pune University (SPPU).
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Nilangekar, K.S., Shravage, B.V. (2018). Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells. In: Turksen, K. (eds) Autophagy in Differentiation and Tissue Maintenance. Methods in Molecular Biology, vol 1854. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_167
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DOI: https://doi.org/10.1007/7651_2018_167
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8748-1
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