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Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells
An overall three-dimensional picture of the distribution of epidermal cells at a given time point is of importance to better characterize epidermal progenitors. We introduce a whole-mount immunofluorescent staining coupled to a multicolor lineage tracing model for analyzing the spatiotemporal organization of epidermal stem cells. Laser scanning confocal microscopy with multiple labelling allows for robust imaging of dorsal skin with excellent resolution.
KeywordsConfocal microscopy Mouse dorsal skin Multicolor lineage tracing model Whole-mount immunofluorescent staining
This work was supported by the Australian Research Council Discovery Project DP130104777 and the National Health and Medical Research Council project grant 1145350.
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