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Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells

Protocol
Part of the Methods in Molecular Biology book series

Abstract

An overall three-dimensional picture of the distribution of epidermal cells at a given time point is of importance to better characterize epidermal progenitors. We introduce a whole-mount immunofluorescent staining coupled to a multicolor lineage tracing model for analyzing the spatiotemporal organization of epidermal stem cells. Laser scanning confocal microscopy with multiple labelling allows for robust imaging of dorsal skin with excellent resolution.

Keywords

Confocal microscopy Mouse dorsal skin Multicolor lineage tracing model Whole-mount immunofluorescent staining 

Notes

Acknowledgements

This work was supported by the Australian Research Council Discovery Project DP130104777 and the National Health and Medical Research Council project grant 1145350.

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.The University of Queensland, UQ Diamantina Institute, Translational Research InstituteBrisbaneAustralia

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