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Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells

Protocol
Part of the Methods in Molecular Biology book series

Abstract

Primordial germ cells (PGCs), the precursors of gametes, are the only cells capable of acquiring totipotency upon fertilization, but the molecular mechanisms regulating germ cell characteristics have not been fully elucidated. Although intracellular metabolic status and regulation are responsible for the control of cell function and differentiation, little is known about the metabolic features of PGCs. Here, we describe use of an integrated metabolomic, proteomic, and energy metabolic analysis method to comprehensively elucidate the metabolic characteristics of PGCs using mass spectrometry.

Keywords

Glycolysis Metabolome Oxidative phosphorylation Primordial germ cells Proteome 

Notes

Acknowledgments

Y.M. was supported by a Grant-in-Aid for Scientific Research (KAKENHI) in the Innovative Areas, “Mechanisms regulating gamete formation in animals” (grant #25114003) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by AMED-CREST (grant #JP17gm0510017h) from the Japan Agency for Medical Research and Development.

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer (IDAC)Tohoku UniversitySendaiJapan
  2. 2.Graduate School of Life SciencesTohoku UniversitySendaiJapan
  3. 3.The Japan Agency for Medical Research and Development-Core Research for Evolutional Science and Technology (AMED-CREST)TokyoJapan
  4. 4.Center for Regulatory Epigenome and DiseasesTohoku University School of MedicineSendaiJapan

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