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Mitophagy pp 173-175 | Cite as

Dopaminergic Neuron-Specific Autophagy-Deficient Mice

  • Shigeto Sato
  • Nobutaka Hattori
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1759)

Abstract

None of the current genetic Parkinson’s disease (PD) models in mouse recapitulates all features of PD. Additionally, only a few of these models develop mild dopamine (DA) neurodegeneration. And the most parsimonious explanation for the lack of DA neurodegeneration in genetic PD models is a compensatory mechanism that results from adaptive changes during development, making it hard to observe the degenerative phenotype over the life span of mice. Here, we characterize DA neuron-specific autophagy-deficient mice and provide in vivo evidence for Lewy body formation. Atg7-deficient mice demonstrate typical Lewy pathology, including endogenous synuclein and neuronal loss, which resembles PD. Furthermore DA levels are affected by dopaminergic neuronal loss. The age-related motor dysfunction and pathology in DA neurons suggest that impairment of autophagy is a potential mechanism underlying the pathology of PD.

Keywords

Atg7 Autophagy Dopaminergic neuron Lewy body Mouse model Parkinson’s disease 

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Department of NeurologyJuntendo University School of MedicineTokyoJapan

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