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pp 1-13 | Cite as

Interactions Between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells

  • Krithika Badarinath
  • Abhik Dutta
  • Akshay Hegde
  • Neha Pincha
  • Rupali Gund
  • Colin Jamora
Protocol
Part of the Methods in Molecular Biology book series

Abstract

The interplay of immune cells and stem cells in maintaining skin homeostasis and repair is an exciting new frontier in cutaneous biology. With the growing appreciation of the importance of this new crosstalk comes the requirement of methods to interrogate the molecular underpinnings of these leukocyte–stem cell interactions. Here we describe how a combination of FACS, cellular coculture assays, and conditioned media treatments can be utilized to advance our understanding of this emerging area of intercellular communication between immune cells and stem cells.

Keywords

Coculture Conditioned media Explants FACS Immune cells Skin Stem cells 

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Krithika Badarinath
    • 1
    • 2
  • Abhik Dutta
    • 1
  • Akshay Hegde
    • 1
    • 3
  • Neha Pincha
    • 1
    • 4
  • Rupali Gund
    • 1
    • 5
  • Colin Jamora
    • 1
  1. 1.IFOM-inStem Joint Research Laboratory, Centre for Inflammation and Tissue HomeostasisInstitute for Stem Cell Biology and Regenerative MedicineBangaloreIndia
  2. 2.National Centre for Biological SciencesBangaloreIndia
  3. 3.Shanmugha Arts, Science, Technology and Research Academy (SASTRA) UniversityThanjavurIndia
  4. 4.Manipal Academy of Higher EducationManipalIndia
  5. 5.Department of DermatologyColumbia UniversityNew YorkUSA

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