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Interactions Between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells

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Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

The interplay of immune cells and stem cells in maintaining skin homeostasis and repair is an exciting new frontier in cutaneous biology. With the growing appreciation of the importance of this new crosstalk comes the requirement of methods to interrogate the molecular underpinnings of these leukocyte–stem cell interactions. Here we describe how a combination of FACS, cellular coculture assays, and conditioned media treatments can be utilized to advance our understanding of this emerging area of intercellular communication between immune cells and stem cells.

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Author information

Authors and Affiliations

  1. IFOM-inStem Joint Research Laboratory, Centre for Inflammation and Tissue Homeostasis, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, Karnataka, India

    Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund & Colin Jamora

  2. National Centre for Biological Sciences, Bangalore, Karnataka, India

    Krithika Badarinath

  3. Shanmugha Arts, Science, Technology and Research Academy (SASTRA) University, Thanjavur, Tamil Nadu, India

    Akshay Hegde

  4. Manipal Academy of Higher Education, Manipal, Karnataka, India

    Neha Pincha

  5. Department of Dermatology, Columbia University, New York, NY, USA

    Rupali Gund

Authors
  1. Krithika Badarinath
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  2. Abhik Dutta
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  3. Akshay Hegde
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  4. Neha Pincha
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  5. Rupali Gund
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  6. Colin Jamora
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Corresponding author

Correspondence to Colin Jamora .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Badarinath, K., Dutta, A., Hegde, A., Pincha, N., Gund, R., Jamora, C. (2018). Interactions Between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_155

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  • DOI: https://doi.org/10.1007/7651_2018_155

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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