pp 1-10 | Cite as

Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor

  • Meilang Xue
  • Suat Dervish
  • Christopher J. Jackson
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Skin epidermis is a continuous self-renewal tissue maintained by interfollicular epidermal stem cells (IESCs) that reside in the basal layer of epidermis. IESCs also contribute to the repair and regeneration of the epidermis during wound healing. The great plasticity and easy accessibility afforded by IESCs make them a promising source of stem cells for scientific research and clinical applications. Thus, simple methods to isolate and define pure and viable IESCs are a valuable resource. Here, we provide a method for isolating IESCs from human skin epidermis. This method relies exclusively on selecting cells with a higher expression of the endothelial protein C receptor, using fluorescence-activated cell sorting.

Keywords

Endothelial protein C receptor Epidermal stem cell Fluorescence-activated cell sorting Isolation Skin 

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Meilang Xue
    • 1
  • Suat Dervish
    • 2
  • Christopher J. Jackson
    • 1
  1. 1.Sutton Arthritis Research Laboratory, Kolling Institute of Medical ResearchThe University of Sydney at Royal North Shore HospitalSt LeonardsAustralia
  2. 2.The Westmead InstituteThe University of SydneyWestmeadAustralia

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