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Skin Stem Cells pp 257–266Cite as

Melanoblasts as Multipotent Cells in Murine Skin

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

Melanoblasts (MBs) are melanocyte precursors that are derived from neural crest cells (NCCs). We recently demonstrated the multipotency of MBs; they differentiate not only into pigmented melanocytes but also other NCC derivatives. We herein describe methods for the isolation of MBs from mouse skin by flow cytometry. Methods to culture isolated MBs that retain their multipotency and isolation methods for MBs using gene-modified mouse are also described.

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Acknowledgements

This study was supported by a grant from the program Grants-in-Aid for Scientific Research (C) from the Japan Society for Promotion of Science.

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Authors and Affiliations

  1. Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan

    Tsutomu Motohashi & Takahiro Kunisada

Authors
  1. Tsutomu Motohashi
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  2. Takahiro Kunisada
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Corresponding author

Correspondence to Tsutomu Motohashi .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Motohashi, T., Kunisada, T. (2018). Melanoblasts as Multipotent Cells in Murine Skin. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_144

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  • DOI: https://doi.org/10.1007/7651_2018_144

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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