pp 1-18 | Cite as

Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro

Protocol
Part of the Methods in Molecular Biology book series

Abstract

Coordinated regeneration of melanocyte stem cells (McSCs) and hair follicle stem cells (HSCs) contributes to generation of pigmented hairs. Synchronous regeneration of McSCs with activation of HSCs occurs not only during initiation of a new hair cycle in vivo but also during reconstitution of hair follicles in vitro. The duration of the quiescent state of these stem cells becomes longer and longer in lifespan of mammals, leading to a decreased regenerative ability to form hair follicles. Here, we describe methods to activate McSCs during hair follicle regeneration in vivo, and isolate melanocytes from neonatal mouse skin to generate an immortalized cell line of melanocyte progenitors in vitro, aiming to use them for studying melanogenesis and future clinical application.

Keywords

Hair cycle Hair follicle stem cells Melanocyte stem cells Melanogenesis Regeneration Skin 

Notes

Acknowledgements

Mingxing Lei is supported by the Higher Education Enhancement Program, China Medical University, Projects Funded by China Postdoctoral Science Foundation (2016M590866), Fundamental Research Funds for the Central Universities (106112015CDJRC231206), Special Funding for Postdoctoral Research Projects in Chongqing (Xm2015093), and a fellowship from the China Scholarship Council (2011605042). Ke Yang was supported by National Natural Science Foundation of China (81371718). Weiming Qiu is supported by National Natural Science Foundation of China (81602782) and Natural Science Foundation of Hubei Province (2016CFB348). The authors thank Dr. T.-C. He at the University of Chicago for gifting retrovirus vector and technical assistance.

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Ke Yang
    • 1
  • Weiming Qiu
    • 2
  • Pei-Rong Gu
    • 3
  • Mingxing Lei
    • 3
    • 4
  1. 1.Department of Pediatric Research Institute, Chongqing Engineering Research Center of Stem Cell TherapyChildren’s Hospital of Chongqing Medical University, and Key Laboratory of Child Development and Disorders of Ministry of EducationChongqingChina
  2. 2.Department of DermatologyWuhan General Hospital of Chinese People’s Liberation ArmyWuhanChina
  3. 3.Integrative Stem Cell Center, China Medical University HospitalChina Medical UniversityTaichungTaiwan
  4. 4.Institute of New Drug Development, College of Biopharmaceutical and Food SciencesChina Medical UniversityTaichungTaiwan

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