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pp 1-4 | Cite as

Isolation and Cultivation of Skin-Derived Precursors

  • Xiaoxiao Xiaoxiao
  • Shiyang Dong
  • Yaojiong Wu
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Skin-derived precursors (SKPs) have been shown recently to be capable of inducing hair genesis and hair follicle regeneration. Here, we describe a protocol for SKP isolation and culture based on our experience and previous publications.

Keywords

Cultivation Isolation Skin-derived precursor SKPs 

References

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    Ito M et al (2007) Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding. Nature 447(7142):316–320Google Scholar
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    Morris RJ et al (2004) Capturing and profiling adult hair follicle stem cells. Nat Biotechnol 22:411–417Google Scholar
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    Jahoda CA et al (1984) Induction of hair growth by implantation of cultured dermal papilla cells. Nature 311:560–562Google Scholar
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    Biernaskie J et al (2009) SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells. Cell Stem Cell 5(6):610–623Google Scholar
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    Wang X et al (2016) Hair follicle and sebaceous gland de novo regeneration with cultured epidermal stem cells and skin-derived precursors. Stem Cells Transl Med 5(12):1695–1706Google Scholar
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    Toma JG et al (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3:778–784Google Scholar
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    Toma JG et al (2005) Isolation and characterization of multipotent skin-derived precursors from human skin. Stem Cells 23:727–737Google Scholar

Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Graduate School at ShenzhenTsinghua UniversityShenzhenChina

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