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Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells

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Book cover Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

Stem cells that reside in the bulge of adult mouse hair follicles are a leading model of tissue stem cell research. Ex vivo culturing, molecular and cell biological characterizations, as well as genetic manipulation of fluorescence-activated cell sorting-isolated bulge stem cells offer a useful experimental pipeline to complement in vivo studies. Here we describe detailed methods for culturing, immunostaining, live cell imaging, and adenoviral infection of bulge stem cells for downstream applications such as in vitro clonal and in vivo patch assays.

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Authors and Affiliations

  1. Department of Biological Chemistry, School of Medicine, University of California, Irvine, CA, USA

    Daniel Haensel, Melissa A. McNeil & Xing Dai

Authors
  1. Daniel Haensel
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  2. Melissa A. McNeil
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  3. Xing Dai
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Corresponding author

Correspondence to Xing Dai .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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© 2018 Springer Science+Business Media New York

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Haensel, D., McNeil, M.A., Dai, X. (2018). Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_136

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  • DOI: https://doi.org/10.1007/7651_2018_136

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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