Abstract
Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The methodology reported here covers the quantitative assessment of the total nitrogen content of algal biomass and a description of the methodology that underpins the accurate de novo calculation of a dedicated nitrogen-to-protein conversion factor.
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Becker EW (1994) Microalgae—biotechnology and microbiology. Cambridge University Press, Cambridge
Pignolet O, Jubeau S, Vaca-Garcia C, Michaud P (2013) Highly valuable microalgae: biochemical and topological aspects. J Ind Microbiol Biotechnol 40:781–796. https://doi.org/10.1007/s10295-013-1281-7
Lourenço S, Barbarino E (1998) Distribution of intracellular nitrogen in marine microalgae: basis for the calculation of specific nitrogen-to-protein conversion factors. J Phycol 811:798–811
Templeton DW, Laurens LML (2015) Nitrogen-to-protein conversion factors revisited for applications of microalgal biomass conversion to food, feed and fuel. Algal Res 11:359–367. https://doi.org/10.1016/j.algal.2015.07.013
Koru E (2012) Earth food Spirulina (Arthrospira): production and quality standarts. In: El-Samragy Y (ed) Food addit. InTech, Rijeka, pp 191–203
Garcia MDC, Lopez CVG, Fernandez FGA et al (2010) Protein measurements of microalgal and cyanobacterial biomass. Bioresour Technol 101:7587–7591. https://doi.org/10.1016/J.Biortech.2010.04.077
Servaites JC, Faeth JL, Sidhu SS (2012) A dye binding method for measurement of total protein in microalgae. Anal Biochem 421:75–80. https://doi.org/10.1016/j.ab.2011.10.047
Slocombe SP, Ross M, Thomas N et al (2013) A rapid and general method for measurement of protein in micro-algal biomass. Bioresour Technol 129:51–57. https://doi.org/10.1016/j.biortech.2012.10.163
Redinbaugh MG, Turley RB (1986) Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions. Anal Biochem 153:267–271
Laurens LML, Van Wychen S, McAllister JP et al (2014) Strain, biochemistry, and cultivation-dependent measurement variability of algal biomass composition. Anal Biochem 452:86–95. https://doi.org/10.1016/j.ab.2014.02.009
AOAC (1997) Amino acids in feeds. Official Method 994.12
Mosse J (1990) Nitrogen-to-protein conversion factor for ten cereals and six legumes or oilseeds. A reappraisal of its definition and determination. Variation according to species and to seed protein content. J Agric Food Chem 38:18–24. https://doi.org/10.1021/jf00091a004
Lourenço SO, Barbarino E, Marquez UML, Aidar E (1998) Distribution of intracellular nitrogen in marine microalgae: Basis for the calculation of specific nitrogen-to-protein conversion factors. J Phycol 34:798–811
Lourenço SO, Barbarino E, Lavín PL et al (2004) Distribution of intracellular nitrogen in marine microalgae: calculation of new nitrogen-to-protein conversion factors. Eur J Phycol 39:17–32. https://doi.org/10.1080/0967026032000157156
Lourenco SO, Barbarino E, De-Paula JC et al (2002) Amino acid composition, protein content and calculation of nitrogen-to-protein conversion factors for 19 tropical seaweeds. Phycol Res 50:233–241. https://doi.org/10.1046/j.1440-1835.2002.00278.x
AOAC (1990) Protein (Crude) in animal feed: combustion method. Official Method 990.03
Ball MF, Meloni CR, Diaz MJ et al (1966) Nitrogen measurement in biological fluids and bone by an automatic Dumas technique. Anal Biochem 16:343–348
Kjeldahl J (1883) Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern. Zeitschrift fur Anal Chemie 22:366–383
Acknowledgments
This work was supported by the U.S. Department of Energy under Contract No. DE-AC36-08GO28308 with the National Renewable Energy Laboratory. Funding provided by U.S. DOE Office of Energy Efficiency and Renewable Energy Bioenergy Technologies Office. The U.S. Government retains and the publisher, by accepting the article for publication, acknowledges that the U.S. Government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this work, or allow others to do so, for U.S. Government purposes.
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Laurens, L.M.L., Olstad, J.L., Templeton, D.W. (2018). Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor. In: Spilling, K. (eds) Biofuels from Algae. Methods in Molecular Biology, vol 1980. Humana, New York, NY. https://doi.org/10.1007/7651_2018_126
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DOI: https://doi.org/10.1007/7651_2018_126
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