pp 1-16 | Cite as
Methods for Monitoring Autophagy in Silkworm Organs
In holometabolous insects, various larval organs are remodeled by autophagy during metamorphosis. Although moths and butterflies are among the first animal models in which this self-eating process was described, only in recent years autophagy has been analyzed in detail in these insects. In particular, the silkworm Bombyx mori, which represents a well-studied model among Lepidoptera, provides a wide repertoire of cellular and molecular tools useful for studying the occurrence of autophagy and for evaluating its role in postembryonic development. Here, we describe some morphological, biochemical, and molecular methods to monitor autophagy in silkworm organs.
KeywordsAcid phosphatase Atg8 Autophagy Bombyx mori Gene expression Metamorphosis Midgut Silk gland Transmission electron microscopy
This work was partially supported by FAR 2017 (University of Insubria) to GT. Aurora Montali is a Ph.D. student of the “Life Sciences and Biotechnology” course at Università degli Studi dell’Insubria. Daniele Bruno is a Ph.D. student of the “Biotechnologies, Biosciences and Surgical Technologies” course at Università degli Studi dell’Insubria.
- 29.Moss WD (1983) Methods of enzymatic analysis. In: Bergmeyer J, Grassi M (eds) Esterases, glycosidases, lyases, ligases, vol 4. Verlag-Chemie, Weinheim, pp 92–106Google Scholar