Abstract
Telomeres are repetitive genetic materials that protect the chromosomes by capping the ends of chromosomes. Each time a cell divides, telomeres get shorter. Telomere length is mainly maintained by telomerase. This enzyme is present in the embryonic stem cells in high concentrations and declines with age. It is still unclear to what extend there is telomerase in adult stem cells, but considering these are the founder cells to the cells of the all tissues in a body, understanding the telomere dynamics and expression of telomerase in adult stem cells is very important.
Telomere length has been implicated as one of the markers for neoplastic transformation in both in vivo and in vitro studies. During cancerogenesis, telomeres shorten due to high cell turnover and repeats are added by active telomerase or alternative lengthening of telomeres (ALT). This gradual shortening is replication driven and does not necessarily explain the presence of ultrashort telomeres. Ultrashort telomeres are observed when there is a sudden shortening in telomeres not related with cell division and may arise from breaks in telomeres due to oxidative damage and replication slippage.
Universal STELA is an accurate method for evaluation of ultrashort telomeres in hMSC-telo1 cells. Compared to TRF assay, U-STELA is developed to overcome several problems in detecting abrupt telomere shortening in a single chromosome.
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References
Martens UM, Chavez EA, Poon SSS, Schmoor C, Lansdorp PM (2000) Accumulation of short telomeres in human fibroblasts prior to replicative senescence. Exp Cell Res 256:291–299
Serakinci N, Hoare SF, Kassem M, Atkinson SP, Keith WN (2006) Telomerase promoter reprogramming and interaction with general transcription factors in the human mesenchymal stem cell. Regen Med 1(1):125–131
Jiang H, Ju Z, Rudolph KL (2007) Telomere shortening and ageing. Z Gerontol Geriatr 40:314–324
Grach A (2013) Telomere shortening mechanisms. In: Stuart D (ed) The mechanisms of DNA replication. InTech, Rijeca
Hemann MT, Strong MA, Hao LY, Greider CW (2001) The shortest telomere, not average telomere length, is critical for cell viability and chromosome stability. Cell 107(1):67–77
Capper R, Britt-Compton B, Tankimanova M, Rowson J, Letsolo B, Man S, Haughton M, Baird DM (2007) The nature of telomere fusion and a definition of the critical telomere length in human cells. Genes Dev 21(19):2495–2508
Friis-Ottossen M, Bendix L, Kølvraa S, Norheim-Andersen S, De Angelis PM, Clausen OPF (2014) Telomere shortening correlates to dysplasia but not to DNA aneuploidy in longstanding ulcerative colitis. BMC Gastroenterol 14:8
Cherif H, Tarry JL, Ozanne SE, Hales CN (2003) Ageing and telomeres: a study into organ- and gender-specific telomere shortening. Nucleic Acids Res 31(5):1576–1583
Friedrich U, Griese EU, Schwab M, Fritz P, Thon KP, Klotz U (2000) Telomere length in different tissues of elderly patients. Mech Ageing Dev 119(3):89–99
Bendix L, Horn PB, Jensen UB, Rubelj I, Kolvraa S (2010) The load of short telomeres, estimated by a new method, Universal STELA, correlates with number of senescent cells. Aging Cell 9:383–397
Montpetit AJ, Alhareeri AA, Montpetit M, Starkweather AR, Elmore LW, Filler K, Mohanraj L, Burton CW, Menzies VS, Lyon DE, Collins JB, Teefet JM, Jackson-Cook CK (2014) Telomere length: a review of methods for measurement. Nurs Res 63(4):289–299
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Serakinci, N., Cagsin, H., Mavis, M. (2018). Use of U-STELA for Accurate Measurement of Extremely Short Telomeres. In: Turksen, K. (eds) Stem Cells and Aging . Methods in Molecular Biology, vol 2045. Humana, New York, NY. https://doi.org/10.1007/7651_2018_120
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DOI: https://doi.org/10.1007/7651_2018_120
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