Abstract
Stem cells have the potential to differentiate into specialized cell types under specific conditions in vivo or in vitro, which are used to cure many diseases related to aging. Somatic cell nuclear transfer (SCNT) can reprogram differential somatic cells into cloned embryos and embryonic stem cells can be derived from these cloned embryos. Recipient oocytes have healthier mitochondria and can improve the metabolism competence, lessen the ROS damage, and rejuvenate mitochondrial function of aged cells during reprogramming. Here, we describe a protocol to isolate aged somatic cells and reprogram them into embryonic stem cells by SCNT. These stem cells can be used to differentiate into regenerative somatic cells and replace the aged cells.
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Acknowledgement
This work was supported by the National Key Research and Development Program of China, Stem Cell and Translational Research (Grant No.2016YFA0100203).
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Wu, DY., Zhang, X., Miao, YL. (2018). Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer. In: Turksen, K. (eds) Stem Cells and Aging . Methods in Molecular Biology, vol 2045. Humana, New York, NY. https://doi.org/10.1007/7651_2018_118
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DOI: https://doi.org/10.1007/7651_2018_118
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9712-1
Online ISBN: 978-1-4939-9713-8
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