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Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila

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Mitophagy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1759))

Abstract

Mitochondrial quality control is a key process in tissues with high energy demands, such as the brain and muscles. Recent studies using Drosophila have revealed that the genes responsible for familial forms of juvenile Parkinson’s disease (PD), PINK1 and Parkin regulate mitochondrial function and motility. Cell biological analysis using mammalian cultured cells suggests that the dysregulation of mitophagy by PINK1 and Parkin leads to neurodegeneration in PD. In this chapter, we describe the methods to monitor mitochondrial morphology in the indirect flight muscles of adult Drosophila and Drosophila primary cultured neurons and the methods to analyze the motility of mitochondria in the axonal transport of living larval motor neurons.

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Author information

Authors and Affiliations

  1. Department of Treatment and Research in Multiple Sclerosis and Neuro-Intractable Disease, Juntendo University Graduate School of Medicine, Tokyo, 113-8421, Japan

    Tsuyoshi Inoshita & Kahori Shiba-Fukushima

  2. Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine, Tokyo, 113-8421, Japan

    Hongrui Meng

  3. Department of Research for Parkinson’s Disease, Juntendo University Graduate School of Medicine, Tokyo, 113-8421, Japan

    Yuzuru Imai

  4. Department of Neurology, Juntendo University Graduate School of Medicine, Tokyo, 113-8421, Japan

    Yuzuru Imai

  5. Department of Neurology, Juntendo University School of Medicine, Hongo, Bunkyo, Tokyo, Japan

    Nobutaka Hattori

Authors
  1. Tsuyoshi Inoshita
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  2. Kahori Shiba-Fukushima
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  3. Hongrui Meng
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  4. Nobutaka Hattori
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  5. Yuzuru Imai
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Corresponding author

Correspondence to Yuzuru Imai .

Editor information

Editors and Affiliations

  1. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Nobutaka Hattori

  2. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Shinji Saiki

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Inoshita, T., Shiba-Fukushima, K., Meng, H., Hattori, N., Imai, Y. (2017). Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila . In: Hattori, N., Saiki, S. (eds) Mitophagy. Methods in Molecular Biology, vol 1759. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_9

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  • DOI: https://doi.org/10.1007/7651_2017_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7749-9

  • Online ISBN: 978-1-4939-7750-5

  • eBook Packages: Springer Protocols

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