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Purification of Target Proteins from Native Tissues: CCT Complex from Bovine Testes and PP2Ac from Porcine Brains

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Tissue Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1788))

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Abstract

Proteomics is a powerful approach for systematic identification and quantification of the entire proteome of a biological system (cell, tissue, organ, biological fluid, or organism) at specific time points (http://www.nature.com). Extracting and purifying target proteins from native tissues are essential steps for many aspects of proteomic studies. In this chapter, we will introduce the experimental procedures to obtain soluble proteins from two different tissues: (1) the CCT (cpn-containing TCP-1) complex from bovine testes and (2) the protein phosphatase 2A (PP2A) catalytic subunit (PP2Ac or C) from porcine brains. With these two examples, we would like to provide some general guidelines for researchers on how to extract and purify target proteins from specific tissues and extend these approaches to other proteins of interest.

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Acknowledgment

This work was supported by ACS research scholar grant (Y. Xing), R01 GM096060-01 (Y. Xing), and T32 CA009135 (N. Wlodarchak). We would also like to thank Dr. Anming Xiong and Edward H. Wang for proofreading.

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Authors and Affiliations

  1. McArdle Laboratory for Cancer Research, WIMR II 6451, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA

    Feng Guo, Nathan Wlodarchak, Patrick Menden & Yongna Xing

  2. Genencor International (DuPont), Palo Alto, CA, USA

    Feng Guo

Authors
  1. Feng Guo
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  2. Nathan Wlodarchak
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  3. Patrick Menden
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  4. Yongna Xing
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Corresponding author

Correspondence to Yongna Xing .

Editor information

Editors and Affiliations

  1. Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, California, USA

    Minnie M. Sarwal

  2. Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, California, USA

    Tara K. Sigdel

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Guo, F., Wlodarchak, N., Menden, P., Xing, Y. (2017). Purification of Target Proteins from Native Tissues: CCT Complex from Bovine Testes and PP2Ac from Porcine Brains. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_89

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  • DOI: https://doi.org/10.1007/7651_2017_89

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7852-6

  • Online ISBN: 978-1-4939-7854-0

  • eBook Packages: Springer Protocols

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