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Combination Strategy of Quantitative Proteomics Uncovers the Related Proteins of Colorectal Cancer in the Interstitial Fluid of Colonic Tissue from the AOM-DSS Mouse Model

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Tissue Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1788))

Abstract

Quantitative proteome analysis using iTRAQ is an important technique to find disease-related proteins. As an important component of tumor microenvironment, tissue interstitial fluid (TIF) has drawn a great attention for its potential as a source for exploration of the solid tumor biomarkers. On the basis of a mouse model of colorectal cancer (CRC) that was induced by the carcinogenetic reagents, we adopted a quantitative proteome analysis with iTRAQ to discover the CRC-related proteins in the TIFs and with MRM to evaluate the corresponding abundance changes in the individual mouse TIF and serum samples.

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Acknowledgment

This work was supported by the National Key Basic Research Program of China (2011CB910704) and the National Natural Science Foundation of China (81372601).

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Authors and Affiliations

  1. BGI-SHEHZHEN, Yantian District, Shenzhen, Guangdong, China

    Guixue Hou

  2. Beijing Institute of Genomics, Chinese Academy of Sciences, Chaoyang District, Beijing, China

    Yang Wang & Xiaomin Lou

  3. BGI-Shenzhen, Yantian District, Shenzhen, Guangdong, China

    Siqi Liu

Authors
  1. Guixue Hou
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  2. Yang Wang
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  3. Xiaomin Lou
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  4. Siqi Liu
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Corresponding author

Correspondence to Siqi Liu .

Editor information

Editors and Affiliations

  1. Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, California, USA

    Minnie M. Sarwal

  2. Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, California, USA

    Tara K. Sigdel

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Hou, G., Wang, Y., Lou, X., Liu, S. (2017). Combination Strategy of Quantitative Proteomics Uncovers the Related Proteins of Colorectal Cancer in the Interstitial Fluid of Colonic Tissue from the AOM-DSS Mouse Model. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_88

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  • DOI: https://doi.org/10.1007/7651_2017_88

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7852-6

  • Online ISBN: 978-1-4939-7854-0

  • eBook Packages: Springer Protocols

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